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KMID : 0811720060100000325
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Korean Journal of Physiology & Pharmacology
2006 Volume.10 No. 0 p.325 ~ p.0
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CHARACTERIZATION OF IONIC CURRENTS IN HUMAN MESENCHYMAL STEM CELLS FROM UMBILICAL CORD
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Park Kyoung-Sun
Kim Yang-Mi
Chai Young-Gyu
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Abstract
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Mesenchymal stem cells (MSCs) have the capability of renewal and differentiation into various lineages of mesenchymal
tissues. These features of MSCs attract a lot of attention from investigators in the context of cell based therapies of several human diseases. Despite the fact that bone marrow represents the main available source of MSCs, the use of bone marrow-derived cells is not always acceptable due to a high degree of viral infection and a significant drop in cell number and proliferation/differentiation capacity with age. Thus, the search for possible alternative MSC sources is still remained. We isolated MSCs from the umbilical cord pervascular using standard enzymatic method. We describe the isolation of a nonhematopoietic mesenchymal-like stem cell population (CD45- CD34- HLA-DR- CD29+ CD73+ CD105+ CD44+) from human umbilical cord (hUC-MSCs). We characterized the functional ion channels in the cultured hUC-MSCs with whole-cell patch clamp and RT-PCR. Using a KCl pipette solution, three types of outward currents were found in these cells, including the voltage- and Ca2+-activated K+ channel (IKCa), a transient outward K+ current (Ito) and a delayed rectifier K+ current (IKDR). A noise-like IKCa and IKDR were totally suppressed by TEA (10 mM). Moreover, IKCa was sensitively blocked by iberiotoxin (100 nM). Ito was inhibited by 4-aminopyridine (4-AP, 200μM). In addition, two types of inward currents were found in these cells; TTX- and verapamil-sensitive Na+ current (INa) and inward rectifier K+ currents (Kir) were detected among ～30% and ～4% hUC-MSCs, respectively. These results demonstrated that multiple functional ion channel currents (IKCa, IKDR, Ito, INa and Kir) were expressed in hMSCs from umbilical cord, and these functional ion channels which might contribute to physiological cell function. However, the physiological roles of these ion channels remain to be studied.
Source: Korean J Physiol Pharmacol.2006 Oct;10(Suppl II):238
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KEYWORD
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Mesenchymal stem cells, ion channels
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